Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Gene expression alterations of neurotrophins, their receptors and prohormone convertases in a rat model of spinal cord contusion

(2008) Gene expression alterations of neurotrophins, their receptors and prohormone convertases in a rat model of spinal cord contusion. Neuroscience Letters. pp. 261-266. ISSN 0304-3940

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Abstract

We have used a semi-quantitative RT-PCR approach to investigate the alterations in the expression of the main regulators of neuronal survival and death, neurotrophins (NTs), NT receptors, and prohormone convertases (PC), in a rat model of spinal cord contusion. Our results revealed that the expression of the members of NT family (Nerve-Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), and Neurotrophin-3 (NT-3)) is significantly declined in the injured spinal cord, as early as 6 h after the induction of the contusion. The expression was recovered afterward to that of the control levels. Furthermore, the expression of all NTs high-affinity Trk receptors decreased severely after the contusion. While the expression of TrkA and TrkC were completely shut down after 6 and 12 h after injury respectively, the expression of TrkB receptor declined at 12 h after injury and remained at this low level thereafter. In contrast to the pattern of Trk receptor expression, p75NTR receptor showed a significant upregulation after contusion. The expression of PC members functioning in the constitutive secretory pathway, i.e. furin, PACE4 and PC7, increased after damage, while the expression of PC members acting in regulated secretory pathway, PC1 and PC2, reduced after spinal cord injury. All together, the down-regulation of NTs, their designated Trk receptors and PC1/PC2 enzymes along with an upregulation of p75NTR promote neuronal death after injury. Our results suggest that either overexpression of NTs, Trk receptors and PC1/PC2 or interfering with the expression of p75NTR in host and/or grafted cells before transplantation could increase the success of the transplantation. (c) 2008 Elsevier Ireland Ltd. Ali rights reserved.

Item Type: Article
Keywords: neurotrophins Trk receptor p75 receptor pro-protein convertase contusion spinal cord injury marrow stromal cells nerve growth-factor hippocampal-neurons axonal growth injury survival apoptosis bdnf trk proprotein Neurosciences & Neurology
Divisions:
Page Range: pp. 261-266
Journal or Publication Title: Neuroscience Letters
Journal Index: ISI
Volume: 441
Number: 3
Identification Number: https://doi.org/10.1016/j.neulet.2008.06.046
ISSN: 0304-3940
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/7010

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