Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Evaluation of nicotinic receptors agonists and antagonists against paraoxon exposed PC12 cells

(2008) Evaluation of nicotinic receptors agonists and antagonists against paraoxon exposed PC12 cells. Environmental Toxicology and Pharmacology. pp. 22-29. ISSN 1382-6689

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Abstract

Chronic and acute exposure to organophosphate pesticides may lead to persistent neurological and neurobehavioral effects, which cannot be explained by acetylcholinesterase (AChE) inhibition alone. In an attempt to elucidate the mechanism by which paraoxon affects the nicotinic receptors gene expression, the effects of exposure of PC12 cells to 100 mu M concentrations of paraoxon for 48 h in the presence and the absence of nicotinic acetylcholine receptors (nAChRs) agonists and antagonists were characterized. Paraoxon at 100 mu M significantly inhibited AChE activity. On the mRNA level, the alpha(4) and beta(2) subunits of nAChR mRNA were significantly decreased in the cells exposed to paraoxon. On the protein level, alpha(4) and beta(2) subunits of nAChR protein were also significantly reduced. Mecamylamine (10 mu M), dihydro-beta-erythroidine (DH beta E) (5 mu M) and nicotine (10 mu M) efficiently prevented the decrease alpha(4) and beta(2) nAChR mRNA and protein in PC12 cells, but carbamaylcholine a weak agonist of nAChR was not efficient. These observations suggest that alpha(4)beta(2) nAChRs are involved in paraoxon related toxicity and nicotinic receptors antagonists could play some protective role against organophosphate related damages. (C) 2008 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: paraoxon nicotinic acetylcholine receptor mRNA mecamylamine AChE PC12 cells acetylcholine-receptors expression parathion chlorpyrifos subtype systems Environmental Sciences & Ecology Pharmacology & Pharmacy Toxicology
Divisions:
Page Range: pp. 22-29
Journal or Publication Title: Environmental Toxicology and Pharmacology
Journal Index: ISI
Volume: 26
Number: 1
Identification Number: https://doi.org/10.1016/j.etap.2008.01.003
ISSN: 1382-6689
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/7016

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