Abstract

Bacterial resistance to antibiotics is a serious problem and is increasing in prevalence world-wide at an alarming rate. The antimicrobial susceptibility patterns of 1897 gram-positive bacterial Isolates were evaluated. The minimum inhibitory concentration (MIC) of isolates which comprised Staphylococcus aureus (927 isolates), coagulase-negative staphylococci (CNS; 425 isolates), Enterococcus faecalis (320 isolates), Enterococcus faecium (157 isolates), and pneumococci (50 isolates) collected from 3 teaching hospitals in Tehran were determined by agar dilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. The presence of mecA gene was investigated in methicillin-resistant staphylococci by PCR method and vanA and vanB genes were targeted in enterococcal isolates by Multiplex PCR method. The resistance rate to methicillin among S. aureus and CNS isolates were 33 and 49, respectively. All S. aureus isolates were susceptible to vancomycin .The lowest rate of resistance in all S. aureus isolates was found for rifampicin (<4). The vancomycin resistance rate in enterococci isolates was 11 which was more frequent among E. faecium (19) than E. faecalis (4), all resistant isolates carrying vanA. High-level resistance to gentamicin and streptomycin, were detected in 47 and 87 of enterococcal isolates respectively. The rate of penicillin resistance in pneumococci was 3 and about 27 of isolates had reduced susceptibility to penicillin. The prevalence of erythromycin resistant among pneumococci was 58. All pneumococcal isolates were susceptible to ceftriaxone, rifampicin and vancomycin. Our data highlight the importance of access to updated bacterial susceptibility data regarding commonly prescribed agents for clinicians in Iran. © 2009 Tehran University of Medical Sciences. All rights reserved.