(2020) Crocin treatment decreased pancreatic atrophy, LOX-1 and RAGE mRNA expression of pancreas tissue in cholesterol-fed and streptozotocin-induced diabetic rats. Journal of Complementary and Integrative Medicine. ISSN 15533840 (ISSN)
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Abstract
Oxidative stress in diabetic mellitus is a consequence of oxidative stress, which plays a critical role in the pathogenesis of diabetic tissue damage. Receptors for advanced glycation end products and for oxidized low-density lipoproteins (LDL) have critical contribution in oxidative tissue damage. The present study investigated whether anti-diabetic effects of Crocin via modulation of mRNA expression of RAGE and LOX-1 receptors in diabetic rats. In the current study, high-fat cholesterol (HFC) and streptozotocin (40 mg/kg) used to induce type II diabetes. Experimental groups as follows: (Group 1: control); (Group 2: control treatment Crocin); (Group 3: DM STZ); (Group 4: DM treatment STZ + Crocin); (Group 5; DM + HFC STZ + HFC); (Group 6; DM + HFC treatment STZ + HFC + Crocin). Crocin (20 mg/kg/day, i.p.) administered in treatment groups for 60days. Serum glucose and cholesterol levels evaluated on days 5, 30 and 60 after induction of DM. Pancreatic tissue from all group removed on day 60 for histological and RT-PCR analysis. Application of Crocin significantly decreased serum cholesterol levels on day 60 after induction of DM in diabetic + HFC rats. Moreover, Crocin significantly decreased serum glucose levels on days 30 and 60 both in diabetic and diabetic + HFC rats. Crocin partially prevented the atrophic effects of STZ on both exocrine and endocrine parts of pancreas. Additionally, Crocin significantly decreased LOX-1 and RAGE mRNA expression OF pancreas in diabetic rats. The current study suggested that Crocin suppressed atrophic change of the pancreas by decrease of LOX-1 and RAGE mRNA expression in diabetic rats. © 2019 2019 Walter de Gruyter GmbH, Berlin/Boston.
| Item Type: | Article |
|---|---|
| Keywords: | Crocin diabetes mellitus LOX-1 RAGE STZ advanced glycation end product receptor cholesterol glucose messenger RNA oxidized low density lipoprotein receptor 1 carotenoid OLR1 protein, rat streptozocin adult animal cell animal experiment animal model animal tissue antidiabetic activity Article atrophy cell count cholesterol blood level controlled study gene expression glucose blood level histology histopathology male non insulin dependent diabetes mellitus nonhuman pancreas disease pancreas islet pancreas tissue pancreatic atrophy rat reverse transcription polymerase chain reaction upregulation Wistar rat animal disease model drug effect experimental diabetes mellitus genetics metabolism pancreas Animals Carotenoids Diabetes Mellitus, Experimental Disease Models, Animal Rats Rats, Wistar Receptor for Advanced Glycation End Products Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Scavenger Receptors, Class E |
| Journal or Publication Title: | Journal of Complementary and Integrative Medicine |
| Journal Index: | Scopus |
| Volume: | 17 |
| Number: | 2 |
| Identification Number: | https://doi.org/10.1515/jcim-2019-0117 |
| ISSN: | 15533840 (ISSN) |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.bmsu.ac.ir/id/eprint/8297 |
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