Abstract

Urinary tract infections are one of the most common infections in the hospital, which can cause complicity in treatment. The purpose of this study was to determine genotyping patterns based on antibiotic resistance genes and bag isolated from the UTI sample in female and male patients referring to the Urology Department of Baqiyatallah Hospital in 2017-2018. This descriptive study was performed on 100 isolates of Escherichia coli isolated from the urine sample of patients with recurrent urinary tract infection referred to Baqiyatallah Hospital after culture and differential tests. To determine the antibiotic susceptibility, the diffusion method was used to. To determine the susceptibility to the disk, Piperacillin, Cefotaxime, Ofloxacin, Ciprofloxacin, Neurofloxacin, Gentamicin, Nitrofurantoin, Amikacin, Imipenemn, and Meropenem were used. Finally, using the PCR method, the prevalence of antibiotic resistants ESBL (SHV, Ampc, Tem) and aacc3, aac6, and INT) in hospital isolates were investigated. According to the results of this study, it was found that 100 of the strains were resistant to Piperacillin and Cefotaxime, 98 of the strains to Ofloxacin, Ciprofloxacin, and Norrofloxacin, 80 of the strains to Gentamicin, 50 of strains to Nitrofurantoin, 42 of the strains to Amikacin, 40 of strains to Imipenem, and 32 of the samples were resistant to meropenem. Also, according to the results of PCR, antibiotic resistance was found to be due to antibiotic resistance genes in the strains. Due to the potential lack of attention to antibiogram reports, the risk of resistance genes may increase. It is also due to the chronicity of infection and the plasmid resistance genes being highly drug-resistant.