Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Characterization of Biosynthesized Silver Nanoparticles UsingLactobacillus rhamnosusGG and its In Vitro Assessment Against Colorectal Cancer Cells

(2020) Characterization of Biosynthesized Silver Nanoparticles UsingLactobacillus rhamnosusGG and its In Vitro Assessment Against Colorectal Cancer Cells. Probiotics and Antimicrobial Proteins. pp. 740-746. ISSN 1867-1306

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Abstract

Silver nanoparticles are the most desirable nanoparticles broadly used in diverse fields. This study intends to investigate the anticancer properties of synthesized silver/Lactobacillus rhamnosusGG nanoparticles (Ag-LNPs) as a reducing and stabilizing agent in the synthesis process. To prepare silver/Lactobacillus rhamnosusGG nanoparticles, 1 mg/ml cell lysate ofLactobacillus rhamnosusGG and 1 mM silver nitrate solution were mixed and incubated for 72 h. XRD, FTIR, and TEM methods were used for nanoparticle characterization. MTT assay and annexin/PI staining were employed to analyze the toxicity and apoptotic cells levels of Ag-LNPs, respectively. TEM showed that these nanoparticles are spherical shaped about 233 nm in size. FTIR spectroscopy demonstrated that Ag-LNPs were functionalized with biomolecules. XRD pattern showed high purity and face-centered crystal structure of Ag-LNPs. MTT assay revealed that the percentages of HT-29 live cells significantly reduced in the high concentration of Ag-LNPs. Annexin/PI staining showed that these nanoparticles could lead HT-29 cells to apoptosis. This study showed the new Ag-LNP-synthesizing method usingLactobacillus rhamnosusGG as a cost-effective and efficient approach. Also, it showed that these nanoparticles can be considered as a potential active agent for biomedical applications and drug delivery due to their anticancer activities.

Item Type: Article
Keywords: Lactobacillus rhamnosus GG Biosynthesis Colorectal cancer Probiotic green synthesis antibacterial extract cytotoxicity genotoxicity antioxidant prevention anticancer probiotics toxicity Biotechnology & Applied Microbiology Microbiology
Page Range: pp. 740-746
Journal or Publication Title: Probiotics and Antimicrobial Proteins
Journal Index: ISI
Volume: 12
Number: 2
Identification Number: https://doi.org/10.1007/s12602-019-09530-z
ISSN: 1867-1306
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/8786

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