Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Real-Time RT PCR Evaluation of the Xylitol Effect on the Expression of Streptococcus pneumoniae cpsB, cpsD and psaA Genes

(2021) Real-Time RT PCR Evaluation of the Xylitol Effect on the Expression of Streptococcus pneumoniae cpsB, cpsD and psaA Genes. Iranian Journal of Medical Microbiology. pp. 392-399. ISSN 17358612 (ISSN)

[img] Text
Real-Time RT PCR Evaluation of the Xylitol Effect on the Expression of Streptococcus pneumoniae cpsB, cpsD and psaA Genes.pdf

Download (760kB)

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background and Aim: The major pneumococcal pathogenesis factor is the capsular polysaccharide. the production of polysaccharide is regulated by cpsB and cpsD genes. Thus, every agent that induce or inhibition of expression of these genes probably increased pathogenesis of bacteria, intracellular survival and vis versa. The aim of this study was to assay the effect of Xylitol on Streptococcus pneumoniae cpsB, cpsD, psaA and aroE genes expression by Real Time RT-PCR. Materials and Methods: During 2019 in Baqiyatallah university laboratories, 20 clinical isolates and a reference strain Streptococcus pneumoniae (ATCC 6305) were studied. To assay the effect of Xylitol and fructose، different concentration of these sugars were exposed to BHI Broth media. Thereafter, the bacterial suspension was added to 5 Xylitol, 5Xylitol + 5 fructose and control medium for 3hours. RNA was extracted and cDNA synthesis was carried out. Then, the cpsB, cpsD and psaA genes expression levels were measured. Results: The mean of cpsB gene expression ratio in presence of the 5 Xylitol treated media compared with those in the control media and 5 Xylitol + 5 fructose were 0.022 and 0.027 respectively. The means of the cpsD and psaA genes expression ratio in the 5 Xylitol treated media compared with those in the control media and 5 Xylitol + 5 fructose were 1.659 and 0.056, and 0.633 and 3.294 respectively. Conclusion: Our finding revealed that, the 5 Xylitol inhibit the cpsB gene expression properly. Therefore, it is possible to use of Xylitol for reductive on cpsB gene expression and superficial pneumococcal infections control. © 2021, This is an original open-access article distributed under the terms of the Creative Commons Attribution-noncommercial 4.0 International License which permits copy and redistribution of the material just in noncommercial usages with proper citation.

Item Type: Article
Keywords: Capsular genes RT Real-Time PCR Streptococcus pneumoniae Xylitol
Page Range: pp. 392-399
Journal or Publication Title: Iranian Journal of Medical Microbiology
Journal Index: Scopus
Volume: 15
Number: 4
Identification Number: https://doi.org/10.30699/ijmm.15.4.392
ISSN: 17358612 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/9277

Actions (login required)

View Item View Item